Effects ofYushen Zhuyun Prescription on Ovarian Morphology and Sex Hormone of Rats with Decreased Ovarian Reservation Induced by Tripterygium Glycosides
10.3969/j.issn.1005-5304.2016.06.015
- VernacularTitle:育肾助孕方对雷公藤多苷致卵巢储备下降大鼠卵巢形态和性激素的影响
- Author:
Yuli JIN
;
Tingting ZHANG
;
Huayun XU
;
Xuefei YUAN
;
Xiaosun CAI
- Publication Type:Journal Article
- Keywords:
Yushen Zhuyun Prescription;
ovarian reservation;
inhibin B;
anti Mullerian hormone;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(6):56-59
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effects ofYushen Zhuyun Prescription on ovarian morphology and sex hormone of rats with decreased ovarian reservation (DOR) induced by tripterygium glycosides; To explore its mechanism of action.Methods Tripterygium glycosides solution was used to establish DOR model rats by gastric gavage. 48 female SD rats were randomly divided into blank group, model group, positive medicine group andYushen Zhuyun Prescription low-, medium- and high-dose groups.Yushen ZhuyunPrescription groups were given the low, medium, high dosagesYushen Zhuyun Prescription liquid; positive medicine group were given estradiol valerate liquid; model group and blank control group were given normal saline with the same amount, by gavage 2 times per day for 14 consecutive days. The general condition of the rats was observed; ovarian was weighed and the viscera index was calculated; ovarian morphology was observed by HE staining; the levels of serum estradiol (E2), follicle stimulating hormone (FSH) and testosterone (T) were detected by radioimmunoassay; the levels of inhibin B (INHB) and anti Mullerian hormone (AMH) were detected by ELISA.Results Compared with the blank group, ovarian tissue atrophied; the number of follicles was reduced; ovarian index and the level of E2, INHB and AMH decreased; the levels of FSH and LH increased in the model group (P<0.01). Compared with the model group, ovarian tissue morphology improved significantly; the number of follicle and corpus luteum increased; follicular atresia was reduced; ovarian index and the levels of E2, INHB, and AMH increased; the levels of FSH and LH decreased in Yushen Zhuyun Prescription high-dose group (P<0.01). INHB and AMH had significant correlation (P<0.01). ConclusionYushen Zhuyun Prescription can regulate hormone levels, promote the growth and secretion of follicle, and inhibit follicular atresia, thereby improve ovarian reserve function.
