Content Determination of Total Iridoid Compounds and Baldrinal and 11-ethoxyviburtinal from Valerianae Jatamansi Rhizoma et Radix
10.3969/j.issn.1005-5304.2016.06.023
- VernacularTitle:蜘蛛香中总环烯醚萜及2个指标性成分baldrinal 和11-ethoxyviburtinal含量测定
- Author:
Ping LI
;
Xingli YAN
;
Zengping GAO
;
Jinbo SHI
;
Beibei YANG
;
Wenqin JI
;
Qingqing MENG
;
Baohua WANG
- Publication Type:Journal Article
- Keywords:
Valerianae Jatamansi Rhizoma et Radix;
UV;
HPLC;
total iridoid compounds;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(6):88-91
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a method for content determination of total iridoid compounds and baldrinal and 11-ethoxyviburtinal from Valerianae Jatamansi Rhizoma et Radix; To determine the contents of total iridoid compounds and baldrinal and 11-ethoxyviburtinal in Valerianae Jatamansi Rhizoma et Radix from three medicinal origins.Methods UV spectrophotometry was applied, 11-ethoxyviburtinal (cyclopentane-pyran-7-formaldehyde, 4-ethoxy methyl) was set as the reference substance, and the content of total iridoid compounds was determined at 288 nm. HPLC method was used to simultaneously determine the contents of baldrinal and 11-ethoxyviburtinal. The HPLC analysis was performed on a Phenomenex Luna C18 column (250 mm×4.6 mm, 5μm). The mobile phase was composed of acetonitrile-water in gradient elution at a flow rate of 0.95 mL/min. The detection wavelength was 288 nm and the column temperature was 30℃.Results The total iridoid compounds, baldrinal and 11-ethoxyviburtinal were in good linearity within the ranges of 2.088–14.616μg/μL, 74.88–224.64μg, and 41.6–249.6μg, respectively. This method was precise, and with good repeatability, stability and recovery rate.Conclusion The method is accurate, simple, rapid, which can be used for the quality control of Valerianae Jatamansi Rhizoma et Radix.
