Establishment of Aortic Regurgitation Model in Chinese Miniature Pigs Under Echocardiography Guidance
10.3969/j.issn.1000-3614.2016.05.018
- VernacularTitle:超声心动图引导建立小型猪主动脉瓣反流模型的方法学研究
- Author:
Yan SUN
;
Jiande WANG
;
Xiaoni LI
;
Li ZHANG
;
Minghui ZHANG
;
Yue TANG
;
Liujun JIA
;
Liang MENG
;
Hao WANG
- Publication Type:Journal Article
- Keywords:
Animal model;
Echocardiography;
Aortic regurgitation
- From:
Chinese Circulation Journal
2016;31(5):495-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the aortic regurgitation model in Chinese miniature pigs under echocardiography guidance. Methods: The animal models were established by following steps: general anesthesia, measuring body weight and then receiving echocardiography examination to exclude aortic valve lesions; carotid artery was exposured by surgery, catheter was sent to aortic sinus with stiff guide wire penetrates and the position of catheter was adjusted to obtain aortic valve damage. The aortic valve injury and regurgitation were evaluated by ultrasound; then the pigs were killed and the heart was taken to observe aortic valve damage. Results: A total of 7 pigs were used including 4 male and 3 female with the mean body weight of (24.7 ± 3.6) kg. Aortic regurgitation model was successfully established in 5 pigs including 1 mild, 1 mild-moderate, 2 moderate, 1 severe aortic valve regurgitation, and 4 were with valve lealfets perforation and 1 with lealfets tearing. Conclusion:①Echocardiography can smoothly guide wire go through aortic valve and make valve damage at different degrees, it is reliable to establish aortic valve regurgitation model in experimental pigs.②Echocardiography may clearly identify the position and degree for aortic valve injury.
