Mechanisms underlying the promotion of woundhealing by bletilla carrying exogenousrecombinant human epidermal growth factor
10.3969/j.issn.2095-4344.2016.20.011
- VernacularTitle:白芨胶载外源性重组人表皮生长因子促进伤口愈合机制
- Author:
Xiao WANG
;
Ping CUI
;
Ran WU
;
Bei WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(20):2964-2971
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:How to promote wound healing is always the research focus of the surgical physicians in the clinic. Recombinant human epidermal growth factor (rhEGF) can effectively promote wound healing. However, as a biological agent, it is easy to be decomposed under normal temperature.
OBJECTIVE:Toexplorethe mechanisms underlying the promotion of wound healing in the back of rabbits by bletila carrying exogenous rhEGF.
METHODS:Model rabbitswith ful-thickness skin defects in the back were treated with bletila carrying exogenous rhEGF (combined treatment group), bletila, rhEGF, or saline (control group).
RESULTS AND CONCLUSION:The time of wound healing was the shortest (P< 0.05) and the wound healing rate was the highest (P< 0.05) in the combined treatmentgroup. On postoperative days 3 and 10, newly formed granulation tissue, capilaries, and colagenous fibersshowed by hematoxylin-eosin staining staining and Masson staining and the strongest immunoreactivity of vascular endothelial growth factor determined by immunohistochemical staining were found in the combined treatment group (P< 0.05). These findings confirm that bletila carrying exogenous rhEGF promotes wound healing by accelerating the forming of granulation tissue, new-born capilaries, and colagenous fibers, and the effects are superior to either of them alone.
