Establishment of reference ranges of T lymphocyte subsets in healthy Zhuang ethnic minority in Guangxi,China:analysis of the influence of gender,age and nationality
10.3760/cma.j.issn.0254-5101.2016.05.010
- VernacularTitle:建立中国广西壮族健康人群外周血T淋巴细胞亚群正常范围值：性别、年龄和民族影响因素的分析
- Author:
Shuaifeng LIU
;
Fuxiong LIANG
;
Zhiyong SHEN
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
T lymphocyte subsets;
CD4;
Reference range;
Zhuang ethnic minority;
Age;
Gender
- From:
Chinese Journal of Microbiology and Immunology
2016;36(5):374-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the normal reference ranges of T lymphocyte subsets in healthy Zhuang ethnic minority across different age/ gender groups in Guangxi for providing the scientific guidance for clinical diagnosis and treatment evaluation of immunodeficiency diseases and viral infections as well as relat-ed researches. Methods Peripheral blood samples were collected from 405(196 male and 209 female) healthy subjects of Zhuang ethnic group including 82 adolescents and 323 adults. Single platform FACSCali-bur flow cytometry analysis was performed to detect the absolute counts of T lymphocyte subsets(CD45+, CD3+,CD4+ and CD8+)in peripheral blood samples. Results Reference ranges of T lymphocyte absolute counts in healthy Zhuang Chinese aged 14 to 59 were 2 377±696,1 503±459,802±253,581±243 and 1. 52±0. 57 for CD45+,CD3+,CD4+,CD8+ and CD4+ / CD8+. Significant differences in CD45+,CD3+ and CD8+ T lymphocytes were found between adolescents and adults(t = 0. 000,P﹤0. 05),but no significant difference in CD4+ T lymphocytes was observed between the two groups of people(t = 0. 373,P﹥0. 05). Significant difference between male and female groups was observed only in the absolute counts of CD8+ T lymphocytes(t= 0. 036,P﹤0. 05). The absolute counts of T lymphocyte subsets were on the decline along with the growth of the age. Conclusion This study indicates that the counts of T lymphocyte subsets vary with ethnic group,age and gender. Therefore,it is important to establish normal reference ranges of T lym-phocyte subsets for local healthy people based on gender and age for accurate evaluation of local patients' im-mune system status and treatment efficacy.
