Simultaneous Determination of Quercetin and Kaempferol inKaempferia Galanga L. by HPLC

VernacularTitle:高效液相色谱法同时测定山柰中槲皮素和山柰素含量
Author: Chuanhua FENG ; Gang LI ; Jing ZHANG ; Lan XU ; Xiaoxuan TAO
Publication Type:Journal Article
Keywords: HPLC; Kaempferia galanga L.; quercetin; kaempferol
From: Chinese Journal of Information on Traditional Chinese Medicine 2016;23(6):77-79
CountryChina
Language:Chinese
Abstract: Objective To establish an HPLC method for the simultaneous determination of quercetin and kaempferol inKaempferia galanga L..Methods ODS2 C18 (5μm, 4.6 mm×150 mm) was used as chromatographic column; methanol-0.4% phosphate (47:53) was the mobile phase; the flow rate was 1 mL/min; column temperature was 30℃; the detection wavelength was 367 nm; the injection volume was 10μL.Results Quercetin showed good linear relationship in the range of 0.016 5–1.65μg (r=0.999 7). The average recovery rate was 96.8%, RSD=2.02%; kaempferol showed good linear relationship in the range of 0.014 6–1.46μg (r=0.999 5). The average recovery rate was 97.3%, RSD=1.77%.Conclusion The method is simple, accurate, and with good reproducibility, which can be used for content determination of quercetin and kaempferol inKaempferia galanga L..