Establishment of a specific gene diagnosis method for Yersinia pestis by quantitative real-time PCR
10.3760/cma.j.issn.2095-4255.2016.02.009
- VernacularTitle:应用实时荧光定量PCR建立鼠疫特异基因诊断技术
- Author:
Shanshan DONG
;
Ying GUO
;
Hongli TAN
;
Wei LI
;
Peng WANG
- Publication Type:Journal Article
- Keywords:
Quantitative real-time PCR;
Yersinia pestis;
Diagnosis,genes
- From:
Chinese Journal of Endemiology
2016;35(2):119-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using quantitative real-time PCR to establish a rapid specific genetic diagnostic technique for Yersinia pestis.Methods ①Four sets of specific probes and primers were designed,which targeted to chromosome genes of YPO0392,YPO1094,YPO2087 and YPO2090,respectively.②The probes and primers were tested for stability and specificity with 40 strains of Yersinia pestis and 47 strains of non-Yersinia pestis of different sources in Yunnan.③Eight positive DNA in Yulong,Yunnan,were tested with the screened probes and primers.Results ①Two sets probes and primers were selected,they were targeting YPO0392 and YPO1094,respectively.②The results were all positive of the eight positive DNA samples tested.Conclusion Two sets of primers and probes are selected for rapid specific diagnosis of Yersinia pestis.
