Identification and phylogenetic relationship of dermatophytes based on RFLP analysis and nucleotide sequence of internal transcribed spacer (ITS)1 in nuclear ribosome DNA.
- Author:
Yeon Hwa CHOI
1
;
Yeong Seon LEE
;
Jae Il YOO
;
Bong Su KIM
Author Information
1. Laboratory of Nosocomial Pathogens, Department of Microbiological Diseases, National Institute of Health 5 Nokbun-Dong, Eunpyung-Gu, Seoul, 122-701, South Korea.
- Publication Type:Original Article
- MeSH:
Arthrodermataceae*;
Base Sequence*;
DNA*;
DNA, Ribosomal;
Microsporum;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length*;
Ribosomes*;
Trichophyton
- From:Journal of the Korean Society for Microbiology
2000;35(1):49-60
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
ITSI-5.8S-ITSII rDNA region was amplified from the reference strains and clinical isolates with ITS1 and ITS4 primers. These primers amplified DNA fragments of 550 bp in Microsporum audouinii and Trichophyton violaceum, 700 bp in Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum, and Trichophyton tonsurans, and 750 bp in Microsporum ferreugineum and Microsporum canis. The restriction enzyme patterns of PCR products digested with 13 restriction enzyme including PstI were distint among the genera, whereas identical in the same species. Examination of the ITS (Internal Transcribed Spacers)1 nucleotide sequence revealed that there was the genetic difference in each genera and species. Phylogenetic relationship among each species showed that the Trichophyton mentagrophytes was more closely related Trichophyton tonsurans than Trichophyton rubrum, and Microsporum gypseum was less related than Microsporum spp.
