Study on the extraction technology and the antioxidant activity of polysaccharides from Allii tuberosi semen

VernacularTitle:韭菜子多糖提取工艺及体外抗氧化活性研究
Author: Bing LIU ; Shihong DING ; Rongshen WANG ; Shoujun ZHANG ; Wanzhong LI
Publication Type:Journal Article
Keywords: Allii tuberosi semen; Polysaccharides; Extraction process; Antioxidant
From: International Journal of Traditional Chinese Medicine 2015;37(12):1109-1113
CountryChina
Language:Chinese
Abstract: Objective To preliminary establish the process condition of extracting polysaccharides from Allii tuberosi semen.Methods The study was carried out with the content of Semen allii tuberosi polysaccharides as evaluation index, the material liquid ratio, extraction time, and extraction times as observation factors, and L9 (34) orthogonal design as extraction craft.The physical and chemical properties and IR spectroscope were used to identify the extract.Results The optimum preparation condition was determined as: using 12 times water, extracting 3 times, and extracting 160 minutes.According to the results of purple ring reaction and infrared spectrum, the extract from Allii tuberosi semen has polysaccharides.From the statistical processing of data we found when polysaccharides from Semen allii tuberosi has 50％ reducing power, the value of EC50 is 10.2 mg/ml.Conclusion The process is reasonable and suitable and can be used for the extraction of polysaccharides from Allii tuberosi semen, and can provide the theoretical basis for the research and application.