Diagnostic value of high-risk human papillomavirus, p16 and Ki-67 detection in the cervical intraepithelial neoplasia

VernacularTitle:高危型人乳头瘤病毒联合p16、Ki-67检测对子宫颈上皮内瘤变的诊断价值
Author: Yu WANG ; Yang DENG ; Qing LI
Publication Type:Journal Article
Keywords: Human papillomavirus; Cervical intraepithelial neoplasia; p16; Ki-67
From: Cancer Research and Clinic 2015;27(12):823-826
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of HPV-DNA and p16,Ki-67 proteins in intraepithelial lesion of cervix (CIN) so as to provide the aluable evidence for the early clinical diagnosis and genetic therapy of the CIN.Methods Hybrid capture2 was used to detect HPV DNA in 35 cases of CIN Ⅰ,44 cases of CIN Ⅱ,and 20 cases of CIN Ⅲ.Immunohistochemical MaxVision was used to research the expression of p16 and Ki-67 protein in CIN Ⅰ,CIN Ⅱ and CIN Ⅲ.The results were analyzed with clinicopathological factors.Results Positive expression rates of HPV-DNA were 31.4 ％ (11/35),63.6 ％ (28/44),85.0 ％ (17/20) in CIN Ⅰ,CIN Ⅱ and CIN Ⅲ,respectively.The expression rates of p16 in the three groups were 65.7 ％ (23/35),97.7 ％ (43/44),100.0 ％ (20/20),there were statistically significant difference (x2 =21.30,P =0.00).The expression rates of Ki-67 in the three groups were 77.1％ (27/35),79.5 ％ (35/44),95.0 ％ (19/20),there were no difference (x2 =3.00,P =0.22).Conclusions HPV infection is closely related to the occurrence and development of CIN.p16,Ki-67 can be used as objective marks in diagnosis of CIN.