Transthyretinin repress retinal microvascular endothelial cells under high glucose and hypoxia environment
10.3760/cma.j.issn.1005-1015.2016.02.011
- VernacularTitle:高糖缺氧环境下转甲状腺素蛋白对视网膜血管内皮细胞的影响
- Author:
Jun SHAO
;
Yong YAO
- Publication Type:Journal Article
- Keywords:
Retinal Vessels/cytology;
Endothelial cells/physiology;
Prealbumin/drug effects
- From:
Chinese Journal of Ocular Fundus Diseases
2016;32(2):159-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore transthyretin (TTR) effect on retinal vascular endothelial cells (hREC) under high glucose and hypoxia environment.Methods hREC and human retinal pigment epithelial cell (hRPEC) were cultured at low-glucose (LG),high glucose (HG) and hypoxia.The glucose concentration was increased from 5.5 mmol/L up to 25 mmol/L,and hypoxia was induced by 200 μmol/L CoCl2.The cells were divided into LG group,LG-hypoxia group,HG group,HG-hypoxia group according to the different cell culture environment.The growth index was detected at 0,4,8,16,24,36,48,60,72 hours after cultured.Furthermore,hREC and hRPEC were also cultured with additional TTR (4 μmol/L),respectively.Then transwell co-culture system was employed to reveal the effects of hRPEC on the growth of hREC.Results At 72 hours after cultured,the growth index of hREC and hRPEC in LG group were increased as compared with LG-hypoxia group and HG group (hREC:F=17.098,22.970;P＜0.05.hRPEC:F=45.442,9.011;P＜0.05);the growth index of hREC and hRPEC were decreased in HG group and HG-hypoxia group (hREC:F=146.184,P＜0.05;hRPEC:F=27.907,P＜0.05).Additionally,hREC could be significantly repressed by added TTR during culture with high concentration of glucose (F=161.430,24.106;P＜0.05).hREC could be significantly increased by added TTR during culture with low concentration of glucose (F =200.486,48.662;P ＜ 0.05).In co-culture process,hRPEC revealed inhibition activity against hREC under both natural and abnormal environment (LG group:F=15.711,P＜ 0.05;LG-hypoxia group:F =45.659,P＜0.05;HG group:F =7.857,P ＜0.05;HG-hypoxia group:F=6.348,P＜0.05).Conclusion Under high glucose and hypoxia environment,the growth of hREC from neovascular could be inhibited by TTR.
