Effect of subretinal injection of retinal pigment epithelial cells for retinal pigment epithelium in mice
10.3760/cma.j.issn.1005-1015.2016.01.014
- VernacularTitle:视网膜下腔注射视网膜色素上皮细胞对小鼠视网膜色素上皮层的影响
- Author:
Liyu ZHAO
;
Xiaoyun KE
- Publication Type:Journal Article
- Keywords:
Retinal pigment epithelium/cytology;
Cells,cultured;
Animal experimentation
- From:
Chinese Journal of Ocular Fundus Diseases
2016;32(1):58-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of subretinal injection of retinal pigment epithelium (RPE) cells for RPE in mice.Methods A total of 30 postnatal day 7 C57BL/6J mice were randomly divided into normal mice group,OIR model group and OIR model cell transplanted group,10 mice in each group.The OIR model was induced in mice of OIR model group and OIR model cell transplanted group.The RPE cells were subretinal injected into the RPE of mice in OIR model cell transplanted group.At 20 days after the injection,the RPE thickness was evaluated by fluorescence microscope.The expression of RPE65,Bestrophin and zonula occludens-1 (ZO-1) were estimated by Western blot and real-time quantitative PCR (RT-PCR).Results The thickness of RPE in OIR model mice was thinner than that in normal mice;the thickness of RPE in OIR model cell transplantation mice was significantly thicker than that in the OIR model mice.The results of Western blot and RT-PCR indicated that the differences of protein (F=8.597,18.864,25.691) and mRNA expression (F=39.458,11.461,34.796) of RPE65,Bestrophin,ZO-1 were statistically significant between OIR model group and OIR model cell transplanted group (P＜0.05).Conclusions Subretinal injection of RPE cells can promote RPE thickening.RPE65 and Bestrophin protein relative expression levels increased,ZO-1 protein relative expression levels reduced;mRNA expression levels of RPE65,Bestrophin and ZO-1 genes increased.
