Effect of hydrogen-rich saline on TLR4/MyD88 signaling pathway in rats with endotoxin-induced acute lung injury
10.3760/cma.j.issn.0254-1416.2016.01.033
- VernacularTitle:富氢液对大鼠内毒素性急性肺损伤时TLR4/MyD88信号通路的影响
- Author:
Xiao SONG
;
Kai WANG
;
Liwei WANG
;
Jianhua WANG
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Toll-like receptor 4;
Myeloid differentiation factor 88;
Respiratory distress syndrome,adult;
Endotoxemia
- From:
Chinese Journal of Anesthesiology
2016;36(1):120-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of hydrogen-rich saline on Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88) pathway in rats with endotoxin-induced acute lung injury (ALI).Methods Forty pathogen-free male Sprague-Dawley rats,aged 3 months,weighing 250-300 g,were randomly divided into 4 groups (n =10 each) using a random number table:control group (group C);ALI group;hydrogen-rich saline group (group H);ALI + hydrogen-rich saline group (ALI + H group).ALI was induced by intratracheal instillation of lipopolysaccharide (LPS) 5 mg/kg.At 1 and 6 h after LPS instillation,0.6 mmol/L hydrogen-rich saline 10 ml/kg was injected intraperitoneally in H2 and ALI + H2 groups,while the equal volume of normal saline was given in the other two groups.At 12 h after LPS instillation,bronchoalveolar lavage fluid (BALF) was collected for determination of the concentrations of tumor necrosis factor-alpha (TNF-α),interleukin-1beta (IL-1β) and IL-6 by enzyme-linked immunosorbent assay.The animals were then sacrificed,and lungs were removed for examination of the pathological changes which were scored and for determination of wet to dry lung weight ratio (W/D ratio),and expression of TLR4,MyD88 and nuclear factor kappa B (NF-κB) (by Western blot).Results Compared with group C,the concentrations of TNF-α,IL-1β and IL-6 in BALF,lung injury score and W/D ratio were significantly increased,and the expression of TLR4,MyD88 and NF-κB was up-regulated in ALI and ALl + Hgroups (P＜0.05).Compared with group ALI,the concentrations of TNF-α,IL-1β and IL-6 in BALF,lung injury score and W/D ratio were significantly decreased,and the expression of TLR4,MyD88 and NF-κB was down-regulated in ALI + H group (P＜0.05).Conclusion Hydrogen-rich saline can reduce endotoxin-induced ALI through inhibiting TLR4/MyD88 signaling pathway in the lung tissues of rats.
