Role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to unventilated lung during one-lung ventilation in rats
10.3760/cma.j.issn.0254-1416.2016.02.031
- VernacularTitle:PI3K-Akt-HIF-1α信号通路在右美托咪定减轻单肺通气大鼠非通气侧肺损伤中的作用
- Author:
Wei ZHANG
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
1-Phosphatidylinositol 3-kinase;
Protein-serinc-threonine kinases;
Hypoxia-inducible factor 1,alpha subunit;
Dexmedetomidine;
Respiration,artificial;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
2016;36(2):242-245
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of PI3K-Akt-HIF-1α signaling pathway in dexmedetomidine-induced reduction of damage to the unventilated lung during one-lung ventilation (OLV) in rats.Methods Forty adult male Sprague-Dawley rats,weighing 250-350 g,were randomly divided into 4 groups (n=10 each) using a random number table:two-lung ventilation TLV) group,OLV + normal saline group (group OLV),OLV + dexmedetomidine group (group D),and OLV + dexmedetomidine +LY294002 group (group DL).Bilateral lungs were ventilated for 2.5 h in group TLV,and the right lung was ventilated for 2 h followed by 0.5 h TLV in the other groups.The rats were sacrificed,the left lung was removed for determination of wet to dry lung weight ratio (W/D ratio),and expression of phosphorylated Akt (p-Akt) and hypoxia-inducible factor-1 alpha (HIF-1α) (by Western blot).Results Compared with group TLV,W/D ratio was significantly increased,the expression of p-Akt was down-regulated,and the expression of HIF-1α was up-regulated in the other three groups (P＜0.05).Compared with group OLV,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-lα was up-regulated in D and DL groups (P＜0.05).Compared with group DL,W/D ratio was significantly decreased,and the expression of p-Akt and HIF-1α was up-regulated in group D (P＜0.05).Conclusion PI3K-Akt-HIF-1α signaling pathway is involved in dexmedetomidine-induced reduction of damage to the unventilated lung during OLV in rats.
