Role of nicotinamide adenine dinucleotide phosphate hydrogen oxidase in bupivacaine-induced injury to neurons
10.3760/cma.j.issn.0254-1416.2016.02.006
- VernacularTitle:NADPH氧化酶在布比卡因诱发神经细胞损伤中的作用
- Author:
Tao ZHANG
;
Huanhuan XIU
;
Hanxin PENG
;
Wenqi HUANG
- Publication Type:Journal Article
- Keywords:
NADPH oxidase;
Bupivacaine;
Drug toxicity
- From:
Chinese Journal of Anesthesiology
2016;36(2):148-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase in bupivacaine-induced injury to neurons.Methods SH-SY5Y cells were seeded in 96-well culture plates at a density of 5× 104 cells/well and randomly divided into 3 groups (n=24each) using a random number table:control group (group C),bupivacaine group (group B),and apocynin (NADPH oxidase inhibitor) + bupivacaine group (group A+B).The cells were cultured in a serum-free medium in group C.The cells were cultured in a serum-free medium containing 1 mmol/L bupivacaine in group B.In group A + B,the cells were cultured for 30 min in a serum-free medium containing apocynin 100 μmol/L,and then cultured in a serum-free medium containing 1 mmol/L bupivacaine.At 2,4 and 6 h of incubation,the cells in 6 wells of each group were selected to evaluate the cell viability by MTS assay.At 4 h of incubation,the cells in 6 wells of each group were selected to detect reactive oxygen species (ROS) level by flow cytometry.Results Compared with group C,the cell viability was significantly decreased at 4 and 6 h of incubation,and the production of ROS was increased at 4 h of incubation in group B (P＜ 0.05).Compared with group B,the cell viability was significantly increased at 4 and 6 h of incubation,and the production of ROS was decreased at 4 h of incubation in group B (P＜0.05).Conclusion NADPH oxidase is involved in bupivacaine-induced injury to neurons.
