Effects of Calf Serum on Human Corneal Epithelial Cells in Vitro.
10.3341/jkos.2011.52.7.852
- Author:
Jong Soo LEE
1
;
Eun Hee KIM
;
Su Jin KIM
Author Information
1. Department of Ophthalmology, Pusan National University School of Medicine, Medical Research Institute, Pusan National University Hospital, Busan, Korea. jongsool@pusan.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Calf serum;
Corneal epithelial cell toxicity;
LDH;
MTT
- MeSH:
Chromatin;
Cytokines;
Cytoplasm;
Epithelial Cells;
Humans;
L-Lactate Dehydrogenase;
Organelles;
Transforming Growth Factor beta1
- From:Journal of the Korean Ophthalmological Society
2011;52(7):852-858
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the biologic effects of topical calf serum on corneal epithelial cells in vitro. METHODS: The effects of calf serum on the corneal epithelial cells were evaluated using the MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, and the concentration of IL-1alpha, TGF-beta1 and MMP-9 in the cells was measured. Cell damage was determined using lactate dehydrogenase (LDH), and cellular morphologies were examined by transmission electromicroscopy. RESULTS: Metabolic activity of the corneal epithelial cells decreased at higher concentrations and longer exposure durations. IL-1alpha, TGF-beta1 and MMP-9 titers were lower in calf serum-treated cells than in the control. LDH and cellular damage to the corneal epithelial cells, such as chromatin margination and cytoplasmic organelle swelling, were prominent in cells treated with 30% calf serum. CONCLUSIONS: Cellular metabolic activity was higher and cellular toxicity was lower in cells treated with 10% calf serum compared to those treated with the 20% and 30% concentrations. Furthermore, inflammatory cytokines were sufficiently inhibited in cells treated with the 10% solution. These results indicate that 10% calf serum could be used clinically.
