The expressions of Caspase-9 and heat-shock protein-90 after focal cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-9026.2015.12.021
- VernacularTitle:大鼠局灶性脑缺血再灌注损伤后半胱氨酸蛋白酶9及热休克蛋白-90的表达
- Author:
Lixin YANG
;
Tao TAO
;
Changlu LUO
;
Liling GU
;
Jian XU
;
Zhi LIU
;
Jing ZHANG
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Caspase-9;
Heat-shock proteins
- From:
Chinese Journal of Geriatrics
2015;34(12):1357-1359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Caspase-9 and heat-shock protein-90 (HSP 90) in rats after focal cerebral ischemia-reperfusion injury.Methods The male SD rats (200-250 g) were divided into three groups by the random number table: normal group, sham group and cerebral ischemia-reperfusion (CIR) group.Each group was sorted into four subgroups including group 6 h, group 24 h, group 48 h and group 72 h according to the reperfusion time.Suture-occluded method was adopted to prepare focal cerebral ischemia-reperfusion(CIR) injury in rat model.Enzymelinked immunosorbent assay (ELISA) method was used to detect the variations of Caspase-9 and HSP-90 expression in rats.Results The changes in Caspase-9 and HSP 90 expression in the brain cells were observed by ELISA method.The expression of Caspase-9 and HSP-90 was weakly expressed in sham group, and was at peak in CIR group within 24 h-48 h, then began to decline at 72 h after the reperfusion time.The differences in the expression of caspase-9 and HSP-70 between sham group and normal group were not statistically significant.Conclusions Apoptotic cells gradually increase along with reperfusion time and reach the peak at 48 h after cerebral ischemia-reperfusion.In ischemia half dark stripe, the expression of Caspase-9 and HSP 90 is increased in neuronal cells after cerebral ischemia-reperfusion, and the positive cells number is at peak at 48 h after cerebral ischemiareperfusion.Apoptosis of neuronal cells after cerebral ischemia and reperfusion is a dynamic evolutionary process.The expression of Caspase-9 and HSP 90 in nerve cells plays an important role in regulating cell apoptosis.
