MicroPET imaging with 2-[18F] fluoropropionic acid in Lewis lung carcinoma-bearing mice
10.3760/cma.j.issn.2095-2848.2016.02.018
- VernacularTitle:Lewis肺癌小鼠2-18F-氟丙酸microPET显像
- Author:
Yonghong DANG
;
Jiong CAI
;
Xin LI
;
Ling WANG
;
Fang LI
- Publication Type:Journal Article
- Keywords:
Propionic acids;
Isotope labeling;
Fluorine radioisotopes;
Tomography,emission-computed;
Tomography,X-ray computed;
Lung neoplasms;
Mice
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2016;36(2):180-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare 2-[18F]fiuoropropionic acid (18F-FPA) and evaluate its biodistribution and imaging capacity in Lewis lung carcinoma-bearing mice.Methods 18F-FPA was prepared by nucleophilic substitution reaction,and its hydrophilicity was analyzed.18F-FPA (7.4-11.1 MBq) was injected into Lewis lung carcinoma-bearing mice via tail vein.MicroPET imaging was performed at 20,80 min after the injection.The biodistribution of 18F-FPA in organs was analyzed.The blocking effects of sodium propionate and dichloroacetate to 18F-FPA were tested in vivo.Data were analyzed by two-sample t test using GraphPad Prism software.Results The synthesis of 18F-FPA took 40 min.18F-FPA had high radiochemical purity (＞99％) and hydrophilicity.18F-FPA was mainly distributed in the carcinoma,the urinary bladder and the caecum.The radioactive uptakes in muscles,brown fat and bones were relatively low.Quantitative analysis showed that the uptake of 18F-FPA in Lewis lung carcinoma from 20 min to 80 min was slightly increased ((17.03±2.87) ％ID/g vs (19.33±2.45) ％ID/g) without significant difference (t=1.100,P＞0.05).Neither sodium propionate nor dichloroacetate could block the uptake of 18F-FPA in Lewis lung carcinoma (t=1.544,0.894;both P＞0.05).Conclusions 18F-FPA can be quickly synthesized and has good physicochemical properties.It can be used as a tracer to visualize Lewis lung carcinoma in mice,and its tumor uptaking can not be blocked by propionate and dichloacetate.18 F-FPA PET has the potential to detect lung cancer noninvasively in clinic.
