Response of potassium channels to estrogen and progesterone in the uterine smooth muscle cells of adenomyosis in vitro
10.3760/cma.j.issn.0529-567x.2015.11.010
- VernacularTitle:子宫腺肌病子宫平滑肌细胞中钾离子通道mRNA的表达及雌、孕激素对其的影响
- Author:
Jinghua SHI
;
Li JIN
;
Jinhua LENG
;
Jinghe LANG
- Publication Type:Journal Article
- Keywords:
Endometriosis;
Myocytes,smooth muscle;
Potassium channels;
Estrogens;
Progesterone;
In vitro;
Cells,cultured;
Adenomyosis
- From:
Chinese Journal of Obstetrics and Gynecology
2015;50(11):843-847
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of potassium channels and the influence of estrogen and progesterone on the cultured uterine smooth muscle cells (USMC) of adenomyosis in vitro.Methods There were 22 cases of adenomyosis hysterectomy in the adenomyosis group and 12 patients with cervical intraepithelial neoplasia Ⅲ removal of the uterus in the control group.USMC were separated and cultured in vitro, incubated with different concentrations of estrogen and progesterone.We used reverse transcription-PCR to dectect the expression of large-conductance calcium-and voltage-sensitive potassium channel α subunit (BKCa α) and voltage-gated potassium channel 4.3 (Kv4.3).Results The mRNA expression of BKCa α and Kv4.3 in the adenomyosis group (4.43 ±2.05 and 4.52± 1.97) were significantly higher than those in the control group (0.83±0.25 and 0.86±0.19, P＜0.05).In the control group, Kv4.3 mRNA decreased after treated with 0.1 nmol/L (0.17±0.10) and 1.0 nmol/L (0.13±0.08) estrogen than before (0.55±0.29, P＜0.05).In the adenomyosis group, BKCa α mRNA decreased significantly after treated with 10.0 nmol/L estrogen (0.56±0.27 versus 1.01±0.35, P＜0.05).0.1 μmol/L progesterone elevated both BKCa α mRNA (0.44±0.24 versus 0.16±0.09) and Kv4.3 mRNA (1.29±0.51 versus 0.55±0.29) in the control group (all P＜0.05);however, there were no significant difference in adenomyosis group of different concentration of progestrone (P＞0.05).Conelusuion There is an abnormal expression of potassium channels in the adenomyosis USMC, which is regulated by high concentration of estrogen and might be resistant to progesterone.
