Cdc20 mutation promotes development of colon cancer in APCmin/+ mice
10.3760/cma.j.issn.1006-9801.2016.03.002
- VernacularTitle:Cdc20基因突变促进APCmin/+小鼠结肠癌的发展
- Author:
Juling FENG
;
Lei ZHAO
;
Wen CHEN
;
Hui ZHONG
;
Di XIE
;
Kai YIN
- Publication Type:Journal Article
- Keywords:
Colon neoplasms;
Mice,mutant strains;
Gene,Cdc20;
Gene,APC
- From:
Cancer Research and Clinic
2016;28(3):150-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To demonstrate the relationship between Cdc20 mutation and the promotion of colon cancer via Cdc20loxp/+ APCmin/+ villin-cre+/-compound mutant mice.Methods Cdc20loxp/+ APCmin/+ villin-cre+/-compound mutant mice and APCmin/+ mutant mice were generated by mice mating strategy.The colon tumors of two group mice were compared by phenotypic analysis and histology analysis.Results Phenotypic analysis showed that the number of tumors in Cdc20loxp/+ APCmin/+ villin-cre+/-compound mutant mice group and APCmin/+ mutant mice group was 1.2±0.5 and 1.6±0.5, respectively (t =0.215, P =0.588), and the maximum diameter of tumors was (2.7±0.3) cm and (2.5±0.2) cm, respectively (t =0.568, P =0.575).Pathologic type of Cdc20loxp/+ APCmin/+ villin-cre+/-compound mutant mice was adenocarcinoma, while that of APCmin/+ mice was tubular adenoma.Conclusion Cdc20 carrying a null allele can accelerate the promotion of colon cancer in APCmin/+ mice without influence on the tumor number and size.
