Antimicrobial activities of traditional Chinese medicine aqueous extract on propionibacterium acnes in vitro
10.3760/cma.j.issn.1671-0290.2016.01.016
- VernacularTitle:中药水提物对痤疮丙酸杆菌的体外抑制作用
- Author:
Hengli LIAO
;
Xinyu LIN
;
Xiangning HUANG
;
Hua YU
;
Fang WANG
;
Yichun WANG
- Publication Type:Journal Article
- Keywords:
Propionibacterium acnes;
Traditional Chinese medicine aqueous extract;
Agar dilution method
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2016;22(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of 19 kinds of traditional Chinese medicine (TCM) aqueous extracts in vitro on the standard and clinical strains of propionibacterium acnes.Methods We collected lesion contents of acne vulgaris patients and conducted anaerobic cultivation of propionibacterium acnes at 35 ℃ for forty-eight hours.After tested by oxygen resistance experiment,Gram staining microscopy,catalytic test,nitrate test and sugar fermentation experiment,ANI anaerobic identification card,and VITEK fully-automatic microbe instrument,the strains were identified as propionibacterium acnes.We used AGAR dilution method to test the MIC values of various TCM aqueous extracts.Results The MIC values of Cortex phellodendri,Scutellaria baicalensis,and Rhiubarb were 25 mg/ml,respectively;the MIC values of Sophora flavescens and Honeysuckle were 50 mg/ml;the MIC values of Forsythia was 100 mg/ml.13 kinds of TCM aqueous extract did not produce bacteriostasis at the highest concentration of 200 mg/ml including Belvedere fruit,Chinese bulbul,Hedyotis diffusa,Houttuynia cordata,Purslane,Yerbadetajo herb,Radix isatidis,Lithospermum erythrorhizon,Folium isatidis,Taraxacum,Semen plantaginis,Angelica dahurica,and Fructus cnidii.Conclusions Aqueous extracts of Cortex phellodendri,Scutellaria baicalensis,Rhubarb,Sophora flavescens,Honeysuckle and Forsythia have good inhibitory effects in vitro on Propionibacterium acnes.
