Proliferation, collagen production and related gene expression in keloids and normal skin fibroblasts
10.3760/cma.j.issn.1671-0290.2015.06.013
- VernacularTitle:瘢痕疙瘩成纤维细胞与正常人皮肤成纤维细胞增殖和胶原产生及相关基因的表达
- Author:
Jiang JI
;
Wenya WU
;
Jing JING
;
Yuhua SU
;
Xin SHI
;
Ye TIAN
;
Jianping CAO
- Publication Type:Journal Article
- Keywords:
Keloid;
Fibroblast;
Cell cycle;
Genes;
Pathogenesis
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2015;21(6):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the proliferation,collagen production and related gene expression in keloids and normal skin fibroblast.Methods Isolated primary cells of keloid fibroblasts (KFb,n=12) and normal human dermal fibroblasts (NFb,n=12) were identified,the cell viability and proliferating potential and the cell cycle were detected,and the difference on the collagen synthesis between KFb and NFb were compared.The expression of cell cycle-associated genes such as p21,p16,and p27 was dectected by real-time fluorescent quantitative PCR.Results The phase contrast optical microscopy imaging showed that both KFb isolated from keloid tissues and NFb from normal skin tissues possessed classic and similar fibroblast morphology.But there was a significant difference between cell proliferation,Hyp [(2.30±0.10) μg/ml vs.(1.66±0.13) μg/ml,P＜0.05] and collagen levels [(17.19±0.75) μg/ml vs.(12.37±0.94) μg/ml,P＜0.05].Compared with NFb,KFb exhibited more percentage of G2/M phase cells [(5.90±0.62)％ vs.(16.94 ％±1.93)％,P＜0.05]and less percentage of G0/G1 phase cells [(90.24 ±2.27)％ vs.(75.65±1.92)％,P＜0.05].Cell cycle related genes p16,p21 and p27 were low expressed.Collagen type Ⅰ was highly expressed at mRNA levels in KFb than that in NFb [0.84±0.11,1.32±0.2,1.69±0.12,4.33±0.27 in KFb vs.1.43±0.13,2.56±0.26,2.89±0.37,1.40±0.12 in NFb,P＜0.05].Conclusions There are cell dysfunction and abnormal cellular dynamics in keloid fibroblasts.The formation of keloid likely involves aberrant interactions of some genes that affected its development at different extents.
