Isolation and molecular analysis of blaNDM-1-positive Morganella morganii
10.3760/cma.j.issn.1009-9158.2015.12.015
- VernacularTitle:产NDM-1型碳青霉烯酶摩根摩根菌的分离及分子背景研究
- Author:
Xuan WANG
;
Xiaoyan WU
;
Jiaping LI
;
Guorong SONG
;
Bingfeng QIU
;
Danxia GU
;
Rong ZHANG
- Publication Type:Journal Article
- Keywords:
Morganella morganii;
beta-Lactamases;
Drug resistance,bacterial
- From:
Chinese Journal of Laboratory Medicine
2015;38(12):857-860
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular background of the New Delhi-metallo-1 (NDM-1)-producing Morganella morganii.Methods Two carbapenem-resistant M.morganii named 1 and 2 were isolated in the Second Hospital of Jiaxing,Zhejiang on October 4th and 29th,respectively.Antimicrobial susceptibility was determined by agar dilution method.Pulsed-field gel electrophoresis (PFGE) was performed to analyse the homololgy of isolates.Amplification with specific primers,DNA sequencing,conjugation experiments and genetic environment analysis were conducted to investigate the molecular mechanisms of resistance.Results The two M.morganii isolates were resistant to carbapenem and fluoroquinolones,while susceptible to aztreonam.PFGE analysis indicated that the two isolates were distinguishable.Amplification and DNA sequencing confirmed the coexistence of blaNDM-1,blasHv-12,qnrS1 and aac(6')-Ib-cr in both isolates.Transconjugants were detected with blaNDM.1 and qnrS1 simultaneously.Genetic environment analysis demonstrated that the blaNDM-1-bleMBL-trpF-dsbC-cutA1 structure was in consistence with those from known blaNDM-1-carrying Klebsiella pneumoniae.Conclusion The blaNDM-1 in M.morganii isolates possiblely obtained from K.pneumoniae through translatable plasmids.