The application of GeXP based multiplex RT-PCR assay for respiratory viral infection in children
10.3760/cma.j.issn.1009-9158.2015.12.014
- VernacularTitle:GeXP多重RT-PCR技术在儿童呼吸系统病毒感染病原体检测中的应用
- Author:
Le WANG
;
Mengchuan ZHAO
;
Zhongren SHI
;
Guixia LI
;
Weiwei GUO
;
Shuo YANG
;
Tianxiao YU
- Publication Type:Journal Article
- Keywords:
Respiratory tract infections;
Virus diseases;
Reverse transcriptase polymerase chain reaction;
Electrophoresis,capillary;
Child
- From:
Chinese Journal of Laboratory Medicine
2015;38(12):852-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect twenty types/subtypes of respiratory viruses with the GeXP (genomelab genetic analysis system) based mRT-PCR (multiplex reverse transcription-polymerase chain reaction) assay,and discuss its value in pediatric respiratory viral infection.Methods A retrospective study was conducted on a total of 1 305 inpatient children (0-6 years old) admitted to the Department of Respiratory in Children's Hospital of Hebei Province from May 2014 to November 2014.The sputum samples were collected and the viral DNA or RNA was extracted.After examining the specificity and sensitivity,the GeXP-based mRT-PCR assay was performed to test 20 types/subtypes of respiratory viruses.The obtained results were evaluated in comparison with those of fluorescence real-time PCR and direct sequencing.Chisquare test was conducted by SPSS16.0.Results Among 1 305 hospitalized children,the positive rate of respiratory virus was 58.31％ (761/1 305).The positive rate of respiratory syncytial virus (RSV,17.32％,226/1 305) was the highest followed by the parainfluenza virus 3 (PIV-3,16.02％,209/ 1 305),human rhinovirus (HRV,11.95％,156/1 305),adenovirus (ADV,5.06％,66/1 305),human bocavirus (HBoV,3.14％,41/1 305) and the parainfluenza virus 1 (PIV-1,2.07％,27/1 305).Multiple virus infection was found in 92 children (7.05％).The highest positive rate of respiratory virus was observed in 1-2 years old children (83.28％,249/299);the lowest rate was found in 5-6 years old children (37.68％,26/69).Conclusions The proposed GeXP-based mRT-PCR assay possessed the advantage of high throughput,sensitivity,specificity and rapidity in the detection of twenty types/subtypes of respiratory viruses.It provided a thorough investigation of viral pathogens involved in acute respiratory infection thus could satisfy the clinic requirement and provide epidemic data on the disease prevention.
