Evaluation of the Carba NP test to detect carbapenemase-producing Enterobacteriaceae
10.3760/cma.j.issn.1009-9158.2016.02.008
- VernacularTitle:Carba NP试验用于检测肠杆菌科细菌产碳青霉烯酶的评价
- Author:
Yan YANG
;
Jian CHEN
;
Qian SUN
;
Di LIN
;
Jun CHENG
;
Changgui SUN
- Publication Type:Journal Article
- Keywords:
Enterobacteriaceae;
Drug Resistance,Bacterial;
Microbial Sensitivity Tests;
Sensitivity and Specificity;
Diagnostic Techniques and Procedures
- From:
Chinese Journal of Laboratory Medicine
2016;39(2):99-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance of Carba NP test (CNP) for detection of carbapenemases in carbapenem-non-susceptive Enterobacteriaceae.Methods Carbapenemases in Enterobacteriaceae strains isolated from clinical specimens in the 117th hospital of PLA from January 2010 to December 2014 were detected by both CNP and modified Hodge test (MHT),and corresponding results were analyzed and compared by Chi-square using SPSS version 19.0.Data acquired from PCR were used as the standard.Results Of the 253 carbapenem-non-susceptive Enterobacteriaceae strains,188 positive strains and 65 negative strains were detected by MHT,and 175 positive strains,77 negative strains and 1 undetectable strain were detected by CNP,while 177 positive strains and 76 negative strains were detected by PCR.Sensitivities of two tests were comparable [CNP,98.9％ (175/177),versus MHT,98.3％ (174/177);x2 =0.5,P =0.48],but CNP was more specific [100.0％ (76/76) versus 81.6％ (62/76);x2 =12.1,P ＜ 0.05].Positive result in the CNP was emerged earliest in a KPC-producing strain.Conclusion The CNP is rapid and simple,which have good sensitivity and specificity.Yet further perfection is still needed in the future.
