Effects of a novel synthetic immunostimulator CH2b on iNKT cells isolated from patients with active rheumatoid arthritis
10.3760/cma.j.issn.0254-5101.2015.12.011
- VernacularTitle:免疫调节剂CH2b对活动期类风湿关节炎患者iNKT细胞免疫调节功能的影响
- Author:
Ming MENG
;
Fei YANG
;
Minghua XU
;
Dan CHEN
;
Minghui HOU
;
Jialin LIU
;
Dongzhi CHEN
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis (RA);
Invariant nature killer T (iNKT) cell;
T-bet;
GATA-3
- From:
Chinese Journal of Microbiology and Immunology
2015;35(12):916-920
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of a novel synthetic immunostimulator CH2b containing thiazolidin-4-one on the function of invariant nature killer T (iNKT) cells isolated from patients with active rheumatoid arthritis (RA).Methods Peripheral blood mononuclear cells (PBMCs) isolated from patients with active RA were in vitro cultured with α-Galcer and IL-2.The iNKT cells were separated by using magnetic activated cell sorting (MACS) method.The effects of CH2b on the proliferation of iNKT cells were analyzed by using MTT assay.MILLIPLEX MAP Human Cytokine/Chemokine kit was used to measure the levels of IFN-γ and IL-4 in the supernatants of iNKT cell culture.The expressions of IFN-γand IL-4 at mRNA level in iNKT cells were analyzed by RT-PCR.Western blot assay was used to detect the levels of T-bet and GATA-3 in iNKT cells.Results CH2b significantly enhanced the proliferation of IL-2 activated iNKT cells isolated from the patients with active RA.CH2b promoted the secretion of IL-4,resulting in a decrease in the ratio of IFN-γ/IL-4.Moreover,CH2b promoted the expressions of GATA-3 and IL-4 at mRNA level in iNKT cells.Conclusion The novel immunostimulator,CH2b,might enhance the immunoregulatory effects of iNKT cells by promoting the GATA-3 pathway-mediated secretion of Th2-1ike cytokines and inducing the differentiation of Th0 to Th2 cells.
