Interleukin 10 knockout increases renal fibrosis of ischemia-reperfusion injury model mice

VernacularTitle:白细胞介素10基因敲除加重小鼠肾脏缺血再灌注损伤模型慢性纤维化
Author: Wenjin SUN ; Xin WAN ; Wen CHEN ; Wenjuan HUANG ; Dong SUN ; Changchun CAO
Publication Type:Journal Article
Keywords: Interleukin 10; Ischemia-reperfusion injury; Fibrosis
From: Chinese Journal of Nephrology 2016;32(2):143-148
CountryChina
Language:Chinese
Abstract: Objective To study the effect of interleukin (IL)-10 knockout (IL-10-/-) on renal repair after renal ischemia-reperfusion injury in mice.Methods Eighteen IL-10-/-mice (KO) aged 8-10 weeks and 18 C57BL/6 wild type mice (WT) aged 8-10 weeks were divided into control group (Sham) and renal ischemia-reperfusion injury (IRI) group.The renal tissue morphology change was observed by Hematoxylin and eosin (HE) staining and Masson staining.The expressions of IL-18, Ki67 and TGF-β1 were detected by immunohistochemistry.The expression of TGF-beta1 and IL-18 were detected by Western blotting.Results Compared with that in WT-IRI group, in KO-IRI group renal pathological damage was more severe, renal interstitial fibrosis was visible, Ki67 expression of renal tubular epithelial cells decreased distinctly (P＜0.01), the expression of TGF-betal increased significantly (P＜0.01).Conclusion Repair slows down significantly after kidney ischemia-reperfusion injury and fibrosis occurs gradually in IL-10-/-mice, eventually progressing to chronic kidney disease.