Effect of inhibitor of integrin linked kinase, QLT0267 on tubularepithelial-myofibroblast transdifferentiation of HK-2 Cells
10.3760/cma.j.issn.1001-7097.2016.01.008
- VernacularTitle:整合素连接激酶抑制剂在人近端小管上皮细胞转分化中的作用
- Author:
Lin JIA
;
Zhifeng LIN
;
Li MA
;
Yuling TANG
;
Rui YANG
;
Xiaoping YANG
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Cell transdifferentiation;
Protein-serine-threonine kinase
- From:
Chinese Journal of Nephrology
2016;32(1):50-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and the possible pathway of different concentrations of QLT0267,which was the inhibitor of the integrin-linked kinase (ILK),on the process of high glucose-induced tubularepithelial-myofibroblast transdifferentiation (TEMT) in human renal tubular epithelial cells (HK-2).Methods HK-2 cells were exposed to 30 mmol/L GS,and TEMT model was established.After excluding the effect of high osmotic in TEMT,HK-2 cells were divided into 6 groups by different concentrations of GS and QLT0267 for 48 hours.The rate of the cell proliferation was calculated by MTT.The expression of ILK and α-smooth muscle actin (α-SMA) were determined by immunofluorescence and Western blot,and the expression of protein kinase B (AKT),phosphorylated protein kinase B (p-AKT),and E-cadherin were determined by Western blot.Results (1) The expression of ILK,p-AKT,and α-SMA in HK-2 cells were unregulated and the expression of E-cadherin was downregulated for 48 hours with glucose treating vs control (P ＜ 0.05);(2) The proliferation rate in high glucose group was higher than the group which concentration of QLT0267 was greater than 5 μmol/L (P ＜ 0.05);(3) With the concentrations of QLT0267 increased,the expression of p-AKT,α-SMA was gradually decreased (all P ＜ 0.05),and the expression of E-cadherin was gradually increased (all P ＜ 0.05).Conclusions 30 μmol/L of GS can lead to TEMT in HK-2 cell.The QLT0267 with concentration greater than 5 μmol/L may prevent the activation of ILK downstream proteins,then partially inhibits cell proliferation and TEMT in HK-2 cell.
