Lxn inhibits the proliferation of PANC-1 CD133 positive cells by regulating bcl-2 and bax expression
10.3760/cma.j.issn.1007-631X.2016.01.014
- VernacularTitle:Lxn对CD133+PANC-1胰腺癌细胞增殖及bcl-2、bax表达的影响
- Author:
Zhiqiang ZHENG
;
Jihang ZHENG
;
Zhenzhai CAI
;
Cheng WANG
;
Xiang ZHOU
;
Zhanxiong XUE
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Cell proliferation;
Lxn
- From:
Chinese Journal of General Surgery
2016;31(1):46-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Lxn on CD133 + PANC-1 pancreatic cancer cells.Methods CD133 + PANC-1 cell were isolated by magnetic activated cell sorting (MACS).The properties of the CD133 + PANC-1 cells and Lxn effects on CD133 + PANC-1 cell proliferation in transplanted tumor in nude mice were determined by floating spheres test and tumor xenograft assays.Cell proliferation was assayed by Cell Counting Kit-8 (CCK-8).The Bcl-2,Bax protein and mRNA expression of CD133 + PANC-1 cells treated by Lxn were analyzed by Western blot and Quantitative real-time PCR (qRT-PCR).Results We successfully isolated the CD133 + PANC-1 cells and cultured in serum free medium,CD133 + PANC-1 cells formed sphere,while CD133-PANC-1 cells grew with adherence slowly and then underwent apoptotic process.CD133 + PANC-1 cells showed high tumorigenic in athymic BALB/c mice.Lxn suppressed the growth of transplanted tumor obviously.Compared with control group [(225.52 ± 34.09) mm3],tumor volume decreased significantly (P ＜ 0.05).Significant reduction in cell proliferation was observed in response to Lxn in PANC-1 CD133 + cells by CCK-8 assay with concentration and time dependent manners (P ＜ 0.05).Treated by Lxn,Bcl-2 expression decreased,Bax expression increased.Conclusions Lxn inhibits the proliferation of CD133 + PANC-1 cells probably through a mechanism down-regualting Bcl-2 and up-regulating Bax.
