Effects of atorvastatin and valsartan on high glucose memory-induced injury to endothelial cell
10.3760/cma.j.issn.1000-6699.2015.12.011
- VernacularTitle:阿托伐他汀和缬沙坦对高糖诱导内皮细胞损伤代谢记忆效应的保护作用
- Author:
Xiang XU
;
Yanlin LIN
;
Hui DING
;
Yangang WANG
- Publication Type:Journal Article
- Keywords:
Atorvastatin;
Valsartan;
Human umbilical vein endothelial cell;
Metabolic memory
- From:
Chinese Journal of Endocrinology and Metabolism
2015;31(12):1055-1059
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of atorvastatin and valsartan on high glucose-induced human umbilical vein endothelial cells (HUVECs) injury.Methods Cultured HUVECs were divided and assigned to 9 groups:normal control group,mannitol control group,high glucose group,low dose atorvastatin group (0.1 μ mol/L),medium dose atorvastatin group (1 μmol/L),high dose atorvastatin group (10 μ mol/L),low dose valsartan group (0.1 μmol/L),medium dose valsartan group (1 μmol/L),and high dose valsartan group (10 μmol/L).H UVECs were pretreated with or without 30 mmol/L glucose plus various concentrations of atorvastatin and valsartan (0.1,1,10 μmol/L) for 16 hours and then incubated with 5.5 mmol/L glucose for 6 days.The levels of vascular cell adhesion molecule 1 (VCAM-1),monocyte chemotactic protein 1 (MCP-1),and plasminogen activator inhibitor 1 (PAI-1) in the culture supernatant were measured by enzyme-linked immunosorbent assay.Results Compared with normal glucose group,hyperglycemia memory increased the levels of VCAM-1,MCP-1,and PAI-1 (all P＜0.05),which were still maintained at high levels even after withdrawal of high glucose.Atorvastatin and valsartan treatment decreased the levels of VCAM-1,MCP-1,and PAI-1 (all P＜0.05).Conclusion Atorvastatin and valsartan may lower the secretion of VCAM-1,MCP-1,and PAI-1,and prevent high glucose memory-induced injury to endothelial cell.
