Prediction and identification of immunodominant B cell linear epitopes in capsid proteins VP1 to VP3 of Coxsackievirus A16
10.3760/cma.j.issn.1674-2397.2015.06.007
- VernacularTitle:柯萨奇病毒A16型衣壳蛋白VP1～VP3的B淋巴细胞线性抗原表位预测与确定
- Author:
Meng GAO
;
Hongxia NI
;
Lian ZHU
;
Jianbo LI
;
Limei GAO
;
Yongneng LUO
- Publication Type:Journal Article
- Keywords:
Coxsackievirus infections;
Epitopes,B-Lymphocyte;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Clinical Infectious Diseases
2015;8(6):543-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify immunodominant B linear cell epitopes in capsid proteins VP1 to VP3 of Coxsackievirus A16 (CVA16) strain YY157.Methods The protean algorithms of bioinformatic software Lasergene were used to analyze antigenicity, hydrophilicity and surface probability of amino acid sequence of CVA16 capsid proteins VP1 to VP3.Multiple regions containing potential lineal B cell epitopes were predicted and their corresponding average indexes were calculated by BepiPred 1.0 Server.Corresponding peptides were synthesized and examined in peptide-enzyme-linked immunosorbent assay (ELISA) individually to check whether it reacted positively or negatively toward sera from children with confirmed CVA16 infection.Results Totally 21 possible B cell linear epitopes were predicted according to their relatively strong antigenicity, hydrophilicity and surface probability.The corresponding synthetic peptides reacted positively with sera of CVA16-infected children in a varying extent.Conclusion Immunodominant B cell linear epitopes of capsid proteins VP1 to VP3 of CVA16 strain YY157 are successfully predicted and confirmed.
