Expression Analysis of c-IAP2 in Ovarian Carcinomas.
- Author:
Chi Heum CHO
1
;
Soon Do CHA
;
Won Ki BAEK
;
Kun Young KWON
;
In Soo BAE
Author Information
1. Department of Obstetrics and Gynecology Keimyung University School of Medicine, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
c-IAP2;
Ovarian carcinoma
- MeSH:
Animals;
Apoptosis;
Blotting, Western;
Cell Death;
Coloring Agents;
Corpus Luteum;
DNA-Directed RNA Polymerases;
Female;
Gynecologic Surgical Procedures;
Ovarian Cysts;
Ovary;
Ovulation;
Ovum;
Suicide
- From:Korean Journal of Obstetrics and Gynecology
2001;44(5):852-857
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Apoptosis or programmed cell death is a normally physiological cell suicide program that is highly conserved among all animals. We previously evaluated overexpression of c-IAP1(Inhibitor of Apoptosis Protein) in ovarian carcinomas compared with normal ovaries. In this study, we demonstrate evidence for the involvement of c-IAP2 in ovarian carcinomas. METHODS: Fresh 9 normal ovaries, 5 benign ovarian cysts and 13 ovarian carcinomas were obtained from routine gynecologic surgeries carried out for benign and malignant ovarian tumors. They were examined for the presence of c-IAP2 by RT-PCR(Reverse Transcriptase Polymerase Chain Reaction), Western blot analysis and immunohistochemical stains. RESULTS: Nine of 14 normal and benign ovarian tumors were negative and 11 of 13 ova rian carcinomas were positive for c-IAP2 by RT-PCR. Positive RT-PCR for c-IAP2 was seen in 11/13 of ovarian carcinomas, a significantly higher percentage than in normal and benign ovarian tumors(5/14). All of these tumors showed strong positive for c-IAP2 by western blot and immunohistochemical staining. Whereas negative RT-PCR for c-IAP2 was seen in 9/14 of normal and benign ovarian tumors, a significantly higher percentage than ovarian carcinomas(2/13). Of these 9 negative samples, 6 had positive Western blot and immunohistochemical stains. There was weak concordance of the result. But expression of c-IAP2 in normal ovarian tissue was localized exclusively in the corpus luteum. Therefore, c-IAP2 may play important role in determining the fate of the follicular destiny. There was no expression in normal ovarian stroma cells for c-IAP2. CONCLUSIONS: These findings suggest that c-IAP2 is expressed in ovarian carcinomas and emerging role in cancer. The c-IAP2 expression has been investigated in the normal ovary, where apoptosis is thought to play an important role in ovulation.
