miR-100 regulates cell growth through targeting FGFR3 in patients with pancreatic cancer
10.3760/cma.j.issn.1007-8118.2016.02.013
- VernacularTitle:微小RNA-100靶向成纤维生长因子受体调控胰腺癌细胞的增殖
- Author:
Meiyuan CHEN
;
Chengyi SUN
;
Chao YU
;
Zhipeng LI
;
Jianxin JIANG
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
microRNA-100;
Fibroblast growth factor receptor 3;
Cell proliferation
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(2):116-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of miR-100 on the proliferation of MIA PaCa-2 and CFPAC-1 cells through targeting fibroblast growth factor receptor 3 (FGFR3).Methods miR-100 expression levels in 17 cancer tissues and 17 nonmalignant tissues were examined by Real-time PCR.The effect of miR-100 overexpression on cell proliferation was examined by CCK-8 assay in vitro.Luciferase assay was used to confirm that miR-100 could directly target FGFR3.Real-time PCR and Western blot were used to examine the expression of FGFR3 in miR-100 overexpressing pancreatic cancer cells.The predicted target gene of miR-100,FGFR3,was downregulated by siRNA,and its effect on cell proliferation was also examined.Cell proliferation was analyzed using CCK-8 and Edu assay.Results miR-100 was lowly expressed in pancreatic cancer tissues (P ＜ 0.05).In pancreatic cancer cells,the transfection of lv-miR-100 was able to upregulate the endogenous expression of miR-100 and inhibit the cell proliferation (P ＜0.05).Luciferase assay showed FGFR3 was the direct target of miR-1O0.FGFR3 was significantly downregulated by overexpressing miR-100 in pancreatic cancer cells (P ＜0.05),and FGFR3 knockdown by specific siRNA exerted the similar effect as miR-100 overexpression (P ＜ 0.05).Conclusions Our study identified a new miRNA regulator,miR-100,and clarified a novel mechanism of how miR-100 regulates cell proliferation in pancreatic cancer.The strategy of overexpressing the tumor suppressor miR-100 may provide a new therapeutic approach for treating patients with pancreatic cancer.
