Establishment of digital polymerase chain reaction-ribotyping and database for Clostridium difficile genotyping
10.3760/cma.j.issn.1000-6680.2016.02.011
- VernacularTitle:艰难梭菌数字化聚合酶链反应-核糖体分型方法及参考图谱的建立
- Author:
Kelin XIAO
;
Zhongxing WANG
;
Tianxiang ZHOU
;
Guangxing MAI
;
Qinning WANG
;
Fanrong KONG
- Publication Type:Journal Article
- Keywords:
Clostridium difficile;
PCR-ribotyping;
Fluorescence capillary gel electrophoresis;
Digital;
Database
- From:
Chinese Journal of Infectious Diseases
2016;34(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a digital polymerase chain reaction (PCR) ribotyping method and database for Clostridium difficile genotyping.Methods Sequencer based fluorescence capillary gel electrophoresis was used,instead of agarose gel electrophoresis,to establish the digital PCR-ribotyping of Clostridium difficile.Forty Clostridium difficile reference strains,consisting of 10 PCR-ribotypes (RT),were genotyped by the new digital PCR-ribotyping method to set-up the database.Results The sequencer based fluorescence capillary gel electrophoresis correctly detected PCR-ribotyping products of the 40 reference strains,and showed as digital figure;significant differences of these digital figures were found between the 10 RT.High similar digital figures were shown in twenty-one RT027 strains,three RT002 strains and two RT014 strains.However,seven RT001 strains were typed as four subtypes,and two RT014 strains as two subtypes,respectively.Conclusion A digital PCR-ribotyping,and a reference database consisting of 10 RT are successfully established.
