Influence ofZhulian acupuncture exciting method on hypoxic ischemic brain damage in rats to oxidative stress
10.3760/cma.j.issn.1673-4246.2016.03.012
- VernacularTitle:朱琏针刺兴奋法对缺血缺氧性脑损伤幼鼠脑组织氧化应激的影响
- Author:
Lirong CHEN
;
Xiaoxia PAN
;
Mingming CHEN
;
Fawen ZHENG
;
Jianhua FAN
;
Yanjing LI
;
Lifu WEI
- Publication Type:Journal Article
- Keywords:
Hypoxia-ischemia,brain;
Brain injuries;
Zhulian acupuncture exciting method;
In rats;
Malondialdehyde;
Nitric oxide;
Glutathione peroxidase
- From:
International Journal of Traditional Chinese Medicine
2016;38(3):238-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observate theZhulian acupuncture exciting method in different time of hypoxia ischemia brain injury in rat brain tissue malondialdehyde (MDA), monoamine oxidase (MAO), nitric oxide (NO) and glutathione peroxidase (GSH-PX) content.Methods 7 days old rats were randomly divided into a excitation method acupuncture group I, a excitation method acupuncture group II, a model group, a sham operation group, a normal control group, 10 rats in each group. Excitation method acupuncture group I and normal control group were given stimulation ofZhulian acupuncture exciting method one type technique from 24 h model manipulation, excitation method acupuncture II group from the beginning of the eighth day given Stimulation ofZhulian acupuncture exciting method one type technique. The sham operation group and the model group were not treated by acupuncture. The animals were sacrificed at the twenty-first day after making the model, determined brain tissue MDA、MAO、NO and GSH-PX Content.Results Compared with the model group, MDA (3.4 ± 0.87 nmol/mgvs. 5.50 ± 1.58 nmol/mg) content decreased in the excitation method acupuncture group I (P<0.05). The NO (12.43 ± 3.47μmol/mgvs. 17.10 ± 5.82μmol/mg) content decreased in the excitation method acupuncture group II (P<0.05). MAO (32.12 ± 11.15 U/mg, 31.01 ± 9.92 U/mgvs. 40.90 ± 11.02 U/mg) content were decreased in both excitation method acupuncture group I and group II (P<0.05), while the GSH-PX (2.61 ± 1.20 U/mg, 2.61 ± 1.37 U/mgvs. 1.43 ± 0.49 U/mg) content were increased (P<0.01). ConclusionZhulian acupuncture exciting method one type technique can decrease the content of MDA, MAO and NO reduce the content of hypoxic-ischemic brain injury in rat brain tissue, increase the content of GSH-PX, promote the removal of immature rats with hypoxic ischemic brain damage brain tissue metabolism, and protect brain function.
