Application value of the detection of HCMV-DNA in urine in the diseases of neonatus
10.3969/j.issn.1673-4130.2016.04.018
- VernacularTitle:尿HCMV-DNA检测在新生儿疾病中的应用价值
- Author:
Ruifen ZHONG
;
Siping LI
;
Liyun LIANG
;
Qingqiu CHEN
;
Huiping HUANG
;
Mianyu HU
;
Xiaomei LU
- Publication Type:Journal Article
- Keywords:
cytomegalovirus;
human cytomegalovirus DNA;
neonatus;
urine
- From:
International Journal of Laboratory Medicine
2016;37(4):475-477
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the value of detecting HCMV-DNA in urine in the diseases of newborn babies ,by comparing the result between HCMV-DNA in urine and HCMV-IgM antibody in blood in different diseases of the newborn babies .Methods The urine and blood samples were collected from 1 520 infants who were in hospital of the neonate department from January 2013 to December 2014 .The HCMV-DNA in urine was examined by fluorescence quantitative polymerase chain reaction(FQ-PCR) .And HCMV-IgM antibody in blood was examined by the method of chemiluminescence(ECL) .Results In the 1 520 cases ,153 had de-tectable HCMV-DNA in their urine samples with a positive rate 10 .07% ,while only 4 cases were positive of the HCMV-IgM anti-body in blood with a positive rate 0 .27% .The two groups was statistically significant difference (P<0 .05) .In the infant diseases of 1 520 cases ,the positive rate of HCMV-DNA in urine was 82 .6% of the hepatitis syndrome while the positive rate of HCMV-IgM antibody in blood was 3 .85% .The positive rate of HCMV-DNA in urine was 8 .95% in the jaundice symptoms ,while the posi-tive rate HCMV-DNA in urine was 4 .62% in the pulmonary infection .Each group of disease was statistically significant difference in the positive rate of HCMV-DNA (P<0 .05) .Conclusion The detection of HCMV-DNA in urine is more beneficial to the diag-nosis of HCM V infection of infants than the detection of HCM V-IgM antibody in blood ,especially in hepatitis syndrome ,neonatal jaundice and pulmonary infection .
