Research on the effect of Flavokawain B on the proliferation and apoptosis of acute T lymphoblastic leukemia cells
10.3760/cma.j.issn.2095-428X.2016.03.005
- VernacularTitle:卡瓦胡椒素B对急性T淋巴细胞白血病细胞增殖、凋亡的作用
- Author:
Wenhao LIN
;
Yanlai TANG
;
Zuhan ZHANG
;
Lina WANG
;
Yu LI
;
Xuequn LUO
;
Libin HUANG
- Publication Type:Journal Article
- Keywords:
T lymphoblastic leukemia,acute;
Flavokawain B;
Proliferation;
Apoptosis
- From:
Chinese Journal of Applied Clinical Pediatrics
2016;(3):181-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Flavokawain B on the proliferation and apoptosis of acute T lymphoblastic leukemia(T -ALL)cells and its preliminary mechanism.Methods After the T -ALL cell lines CEM-C7(sensitive to glucocorticoids)and MOLT -4(resistant to glucocorticoids)cells were treated with different concentrations of Flavokawain B,the influence of Flavokawain B on the growth rate and doubling time of CEM-C7 and MOLT -4 cells was observed by 3 -(4,5 -dimethylthiazol -2 -yl)-5 -(3 -carboxymethoxyphenyl)-2 -(4 -sulfophenyl)-2H -tetrazolium(MTS)assay,and apoptosis was analyzed by using flow cytometry.Furthermore,Wes-tern blot assay was used to detect the expressions of Bim,Bcl -2 and cleaved Caspase -9.At last,the expressions of Bim and Bcl -2 in clinical T -ALL patient samples were also detected by using Western blot assay.Results MTS as-say showed that Flavokawain B significantly inhibited the cellular proliferation of T -ALL cell lines in a dose and time dependent manner(P <0.01 ).Flow cytometry findings revealed that Flavokawain B significantly induced the apoptosis of T -ALL cells in a dose -dependent manner(P <0.001 ).Western blot results indicated that Flavokawain B in-creased the expression of Bim and cleaved Caspase -9,and decreased the expression of Bcl -2 in T -ALL cell lines, which increased Bim and decreased Bcl -2 in clinical T -ALL patients samples,both in a dose -dependent manner. Conclusions Flavokawain B can inhibit the proliferation and induce the apoptosis of T -ALL cells by up -regulating the expression of Bim and down -regulating the expression of Bcl -2 and activating Caspase -9,whether resistant to glu-cocorticoids or not.
