Clinical application of cocktail double enzyme labeled antibody(D2-40/CK19)staining for the diagnosis of lymphatic vessel invasion in papillary thyroid carcinoma
10.3760/cma.j.issn.1000-6699.2016.02.005
- VernacularTitle:D2-40／CK19鸡尾酒双酶标记法在甲状腺乳头状癌淋巴管侵犯诊断中的应用
- Author:
Ping SUN
;
Jiayi WAN
;
Yan XIAO
;
Rongrong ZHANG
- Publication Type:Journal Article
- Keywords:
Lymphatic vessel invasion;
Cocktail double enzyme labeled antibody staining;
CK19;
D2-40;
Papillary thyroid carcinoma
- From:
Chinese Journal of Endocrinology and Metabolism
2016;(2):107-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the relationship between lymphatic vessels invasion and clinical pathological features of papillary thyroid carcinoma ( PTC ) .Methods The expressions of D2-40 and CK19 were examined in the 104 specimens of PTC using immunohistochemical staining with combined monoclonal antibodies and cocktail double enzyme labeled antibody( D2-40/CK19) stainings.The two methods were compared in the diagnosis of PTC metastasis, and the factors affecting lymphatic vessels formation were analyzed.Results The positive rate of lymphatic vessels invasion was 37.5%(39/104) by using immunohistochemical staining with combined monoclonal antibodies and 53.8%( 56/104 ) by cocktail double enzyme labeled antibody ( D2-40/CK19 ) staining ( P<0.05).The lymph node metastasis rate was 83.9%(47/56) in the group with lymphatic vessels invasion, significantly higher than that without invasion 22.9%(11/48, P<0.01).The age of patients, diameter of primary tumor were the influence factors of lymphatic vessels invasion in PTC patients(P<0.05 and P=0.063).Conclusion Cocktail double enzyme labeled antibody ( D2-40/CK19 ) staining is a better method to detect lymphatic vessels invasion in PTC than immunohistochemical staining with combined monoclonal antibodies.
