Effects of Morroniside on Expression of CD34 in Ipsilateral Cortex of Focal Cerebral Ischemia-reperfusion Rats
10.3969/j.issn.1006-9771.2016.01.006
- VernacularTitle:莫诺苷对局灶性脑缺血再灌注大鼠皮层CD34表达的影响
- Author:
Zhimin WANG
;
Fangling SUN
;
Tingting LIU
;
Hua CHENG
;
Benxu XIANG
;
Renping WEI
;
Houxi AI
;
Xin TIAN
;
Zixin ZHU
;
Wenrong ZHENG
;
Yufeng WANG
;
Deyu GUO
;
Wen WANG
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion;
morroniside;
CD34;
angiogenesis
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of morroniside on the expression of CD34 in ipsilateral cortex of rats after focal cerebral isch-emia-reperfusion. Methods 45 male Sprague-Dawley rats were divided into sham group (n=9), ischemia group (n=9), and morroniside groups (low, medium and high dosage groups, n=9). The middle cerebral artery were occluded for 30 minutes, and reperfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg, 270 mg/kg after operation. The expression of CD34 in the isch-emic ipsilateral cortex were detected with immunohistochemistry (n=6) and Western blotting (n=3) 7 days after operation. Results The ex-pression of CD34 increased in the ischemia group compared with the sham group, and further increased in the morroniside groups of high dos-age compared with the ischemia group (F>14.865, P<0.001). Conclusion Morroniside could increase the expression of CD34 in the ischemic ipsilateral cortex after ischemia-reperfusion in rats, which may promote the angiogenesis and neurogenesis after ischemia.
