Near-infrared quantum dots through one-pot aqueous synthesization for bio-imaging and photodynamic therapy of gliomas
10.3969/j.issn.1006-5725.2016.01.010
- VernacularTitle:一锅法合成近红外量子点用于胶质瘤荧光显像与光动力治疗
- Author:
Mingxing LIU
;
Jun ZHONG
;
Ningning DOU
;
Lei XIA
;
Guo GAO
- Publication Type:Journal Article
- Keywords:
Glioma;
Near-infrared;
Bioimaging;
Photodynamic therapy;
Quantum dots
- From:
The Journal of Practical Medicine
2016;32(1):29-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize a quantum dot (QD) to recognize glioma cells for imaging and photodynamic therapy. Methods By one-pot aqueous approach, near infrared-emitting CdTe was produced. After detection of its physicochemical characterizations, RGD was conjugated. Emission images were observed with confocal microscopy. To test its toxicity, CdTe-RGD with various concentrations was separately added into U251 and 3T3 cells for incubation in dark circulation. To test its photodynamic effect, U251 and 3T3 cells were then irradiated for 5 ~ 60 min using 632.8 nm laser. Results The QD (Φ = 3.75 nm, PL peak wavelength =700 nm, PLQY=20%) achieved was a spherical crystal with excellent monodispersity. Under confocal microscope , U251 cells were visualized but 3T3 cells not. In dark circulation, the survival rates of both U251 and 3T3 cells were above 85%. After laser irradiation, the survival rate of U251 cells decreased to (37 ± 1.6)%with the increasing of irradiation time and CdTe-RGD concentration. Conclusion With good physicochemical characterization and low toxicity, CdTe-RGD could be applied in biomedical imaging and photodynamic therapy of gliomas.
