Effects of Deep Electroacupuncture on Cartilage in Knee Osteoarthritis Rabbits
10.3969/j.issn.1006-9771.2016.01.008
- VernacularTitle:深刺加电针对兔膝骨关节炎软骨的影响
- Author:
Nini FU
;
Xuezhi LI
;
Fei LIU
;
Xiaofang XI
;
Yi REN
;
Xiaoguang YANG
;
Yu ZHANG
- Publication Type:Journal Article
- Keywords:
knee osteoarthritis;
electroacupuncture;
deep acupuncture;
apoptosis;
acid-sensing ion channel 1;
p38 mitogen-activated protein kinases;
p53;
rabbits
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(1):38-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of deep electroacupuncture on carlilage tissue in knee osteoarthritis (KOA) rabbits. Meth-ods 40 New Zealand rabbits were randomly divided into normal group (A, n=10) and model group (n=30). The model group was modeled KOA with Hulth-Telhag way, and identified with X-ray. Then they were divided into no-treated group (B, n=10), deep electroacupuncture group (C, n=10) and routine electroacupuncture group (D, n=10) randomly. The groups C and D accepted electroacupuncture since 6 weeks after modeling, for 4 weeks. They were measured with pH of joint fluid, observed structure and pathology of cartilage under transmission electron microscope, detected apoptosis index, and determined the expression of acid-sensing ion channel 1 (ASIC1), p38 mitogen-activated protein kinases (p38MAPK) and p53 with Western blotting, and distribution of ASIC1 with immunohistochemistry in cartilage tissue. Re-sults The pHs of joint fluid from high to low were ranged as the groups A=C>D>B (P<0.01). The cartilage structure was more complete in the groups A, C and D than in the group B. The apoptosis rates from less to more were ranged as the groups A=C
