Blockage of PD-L1/PD-1 pathway for enhancing the chemotherapeutic efficacy of cisplatin
10.3969/j.issn.1006-5725.2016.01.015
- VernacularTitle:阻断PD-L1/PD-1途径增强顺铂化疗效果的实验研究
- Author:
Jie WEI
;
Peng KOU
;
Yangyang LIAN
;
Hong LIANG
;
Lihua YANG
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Cisplatin;
PD-1 antibody;
Neoadjuvant chemotherapy;
Human tumor xenograft model
- From:
The Journal of Practical Medicine
2016;32(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the anti-tumor effect and the influence of antitumor immunity of PD-L1/PD-1 blocked by PD-1 antibody combined with cisplatin. Methods Tumor models were established by injecting TC-1 cells into C57BL/6 mice, and the mice were divided into four groups (n = 4). The tumor growth curves and survival curves were drawn to observe the anti-tumor effect. The tumors were then removed; and the PD-L1 and CD8+ T cells were analyzed by immunohistochemical method. Results The anti-tumor effect was greater in the cisplatin group , PD-1 antibody group , and PD-1 antibody plus cisplatin group than in the control group (P < 0.05). Expression of PD-L1 in the tumor tissues was markedly increased in the cisplatin group and it was obviously decreased in the combination group (P < 0.05). CD8+ T cells decreased in the cisplatin group; and expression of CD8+ T cells was significantly increased the combination group (P < 0.05). Conclusion The anti-tumor effect and anti-tumor immunity of cisplatin are enhanced by blocking PD-L1/PD-1 pathway with PD-1 antibody.
