A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

VernacularTitle:小儿肺炎患者尿液的代谢组学初步研究
Author: Jing LI ; Xiaolei LIU ; Xu ZHAO ; Wei LI ; Yulin DAI ; Hao YUE
Publication Type:Journal Article
Keywords: Childhood Pneumonia; Rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry; Uric acid; Metabonomics
From: Chinese Journal of Analytical Chemistry 2016;(3):451-455
CountryChina
Language:Chinese
Abstract: In this study, the rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry ( RRLC-QTOF/MS ) was used to profile the metabolites of urine samples from Childhood Pneumonia ( CP) patients and healthy controls and find the potential biomarkers which can support evidence to early diagnose and cure the disease. Choose 10 CP patients ( age 47. 72 ± 2. 35 months) and 10 healthy controls ( age 46 . 65 ± 1 . 97 months ) . The urine samples were analyzed by RRLC-QTOF/MS and then the resulting data matrices were analyzed by principal components analysis ( PCA ) to find the potential biomarkers. Urine samples of CP patients were successfully distinguished from those of healthy controls. A total of two significantly changed metabolites have been found and identified as potential biomarkers. It is suggested that the disorder of purine metabolism and amino acid metabolism may play an important role in the mechanism of CP.