Prediction of miR-199a-3p targets gene and its bioinformatics analysis
10.7652/jdyxb201602020
- VernacularTitle:miR-199a-3p 靶基因预测及生物信息学分析
- Author:
Xiaojuan XIE
;
Jingjing PAN
;
Liqiang WEI
;
Wei CHEN
- Publication Type:Journal Article
- Keywords:
miR-1 99a-3p;
bioinformatics;
mammalian target of rapamycin (mTOR);
gene ontology (GO) enrichment analysis;
Kyoto Encyclopedia of Genes and Genome (KEGG)pathway analysis;
bladder cancer;
PI3K-Akt;
MAPK;
proteoglycan
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;(2):244-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide theoretical guidance for further research on the role of miR-1 99a-3p in formation and development of bladder cancer.Methods Mature sequence of miR-1 99a-3p was analyzed;target genes and transcription factors of miRNA-1 99a-3p were predicted,and the target genes were analyzed for gene ontology (GO)enrichment and Kyoto Encyclopedia of Genes and Genome (KEGG)pathway.Then TF-miRNA-mRNA network diagram was constructed.Results Sequences of miR-1 99a-3p were highly conserved in various species.In GO analysis,the target genes of miR-1 99a-3p were enriched in many biological processes,such as regulation of cellular process,regulation of macromolecule metabolic process,and regulation of biological process (P <0.01 ).In KEGG pathway,the target genes were mainly located in bacterial invasion pathway of epithelial cells,ECM-receptor interaction pathway,PI3K-Akt signaling pathway,MAPK signaling pathway,small cell lung cancer pathway,and proteoglycans pathway in the cancer (P <0.05).According to the TF-miRNA-mRNA network diagram,the important genes that might be regulated by miR-1 99a-3p were MYC,SP1,mTOR,NFκB,and NFκB1.Conclusion miR-1 99a-3p may directly target mTOR and participate in the formation and development of bladder cancer through regulating PI3K-Akt-mTOR signaling pathway.
