Application of improved chromosome short-term culture method in the chromosome karyotype analysis of leukemia patients
10.7652/jdyxb201602029
- VernacularTitle:染色体短期培养法的改良及在白血病患者染色体核型分析中的应用
- Author:
Pengyu ZHANG
;
Longjin ZHANG
;
Jing LUO
;
Sheping CHEN
;
Xin MENG
;
Wei TIAN
;
Wanggang ZHANG
;
Fuling ZHOU
- Publication Type:Journal Article
- Keywords:
orthogonal design;
bone marrow;
chromosome karyotype analysis;
chromosome preparation meth-od;
leukemia;
chromosome short-term culture;
colchicines
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;(2):288-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To make the chromosome karyotype analysis of 130 patients with leukemia by using the improved chromosome short-term culture method.Methods We optimized the main factors with a single factor gradient experiment in short-term culture of bone marrow chromosome, including colchicines concentration, duration of action of colchicines,and hypotonic time.On this basis,we conducted the three-factors and three-level orthogonal experiment to achieve improved bone marrow chromosome preparation system,which was later applied in 130 patients with leukemia in our hospital.Results The orthogonal experiment results showed that the optimum conditions were colchicines concentration of 0.07 μg/mL,colchicines action time of 80 min,and hypotonic time of 35 min during the preparation of the bone marrow chromosome.Using this method,the chromosome preparation success rate reached 97.69% and the detection rate of abnormal karyotype reached 82.3% in the chromosome karyotype analysis.Conclusion Bone marrow chromosome preparation system with colchicines concentration of 0.07 μg/mL and colchicines action time of 80 min,and hypotonic time of 35 min is worthy of clinical promotion.
