Verification of the interaction between ASGPR and HBV preS1 protein
10.7652/jdyxb201602030
- VernacularTitle:ASGPR 与 HBV preS1蛋白之间相互作用的验证
- Author:
Xi ZHANG
;
Xiaojing LIU
;
Yunru CHEN
;
Ying KONG
;
Xueliang YANG
;
Feng YE
;
Shumei LIN
- Publication Type:Journal Article
- Keywords:
hepatitis B virus (HBV);
preS1 protein;
asialoglycoprotein receptor (ASGPR);
protein-protein interaction;
mammalian two-hybrid assay;
coimmunoprecipitation
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2016;(2):292-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the interaction between asialoglycoprotein receptor (ASGPR)and hepatitis B virus (HBV)preS1 protein in vivo and in vitro ,and identify ASGPR as a cell-surface receptor for HBV,which could elucidate the molecular mechanism of HBV infection.Methods The preS1-ASGPR interaction was examined in mammalian two-hybrid and coimmunoprecipitation system by strictly following the manufacturer’s instructions.Results ASGPR interacted specifically and directly with the preS1 domain of HBV in vivo and in vitro .Conclusion ASGPR may be a candidate receptor for HBV that mediates further step of HBV entry.
