Determination of Uric Acid Based on Chitosan/Ru(bpy) 2+3/Silica Nanoparticles Electrochemiluminescence Sensor
10.11895/j.issn.0253-3820.150705
- VernacularTitle:基于壳聚糖/Ru（bpy）2+3/SiO2纳米粒子电化学发光传感器用于尿酸的检测
- Author:
Xuemei FAN
;
Shumin WANG
;
Zhejian LI
;
Xingwang ZHENG
- Publication Type:Journal Article
- Keywords:
Electrogenerated chemiluminescence;
Chitosan-Ru( bpy) 2+3 -SiO2 nanoparticles;
Uric acid
- From:
Chinese Journal of Analytical Chemistry
2016;(3):342-347
- CountryChina
- Language:Chinese
-
Abstract:
Chitosan-Ru ( bpy ) 2+3 -SiO2 composite nanoparticles ( CRuS NPs ) were prepared by reverse microemulsion method, and based on the Nafion/MCNT composite membrane technology, CRuS NPs were effectively and steadily immobilize on the surface of a glassy carbon electrode to prepare the electrochemiluminescence sensor for uric acid determination. In 0. 1 mol/L PBS (pH 7. 4) buffer solution, when the actuation duration between uric acid and the modified electrode was 15 min, the electrochemiluminescence showed a good linear relationship to the negative logarithm of uric acid concentration in the range of 1. 0 × 10-10-1. 0 × 10-5 mol/L, the linear equation was IECL=-709. 52-202. 74lgC and the correlation coefficient was 0. 9936 with a detection limit of 6. 0 × 10-12 mol/L. The ECL sensor exhibited excellent repeatability and stability, and the RSD for 11 times determination of 1. 0 × 10-8 mol/L uric acid was 2. 9%. The recovery was 98. 5%-103. 5% in the determination of real Uric acid sample.
