Effect of bisdemethoxycurcumin on the proliferation and apoptosis of melanoma B16-F10 cells
10.3969/j.issn.1006-5725.2016.01.016
- VernacularTitle:双脱甲氧基姜黄素对黑色素瘤B16-F10细胞增殖、凋亡的影响
- Author:
Xiaofei ZENG
;
Ruidong MA
;
Guansheng SHANG
;
Qiang FU
;
Shuping LI
;
Xiaoping HE
- Publication Type:Journal Article
- Keywords:
Bisdemethoxycurcumin;
Melanoma B16-F10 cells;
Fine breeding;
Apoptosis
- From:
The Journal of Practical Medicine
2016;32(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of bisdemethoxycurcumin on the proliferation and apoptosis of melanoma B16-F10 cells. Methods The B16-F10 cells were incubated with bisdemethoxycurcumin for 24 h, and MTT assay was used to detect the proliferation of B16-F10 cell. Flow cytometry was used to detect cell cycle and cell apoptosis. A C57BL/6 mouse melanoma model was established to investigate the effect of bisdemethoxycurcumin on the proliferation of melanoma. Expression of BCL-1 in B16-F10 cells and tissues was detected by western blotting assay. Results bisdemethoxycurcumin could significantly inhibit B16-F10 cell proliferation, induce B16-F10 cell apoptosis and block the cell cycle at S phase. The intravenous dosing of bisdemethoxycurcumin could inhibit the growth of melanoma. Bisdemethoxycurcumin could inhibit the expression of BCL-1. Conclusion Bisdemethoxycurcumin can inhibit the proliferation of B16-F10 cell, resulting from its role in promoting cell apoptosis.
