Research on the Cytotoxicity of the Heterogeneous Acellular Corneal Stromal Leaching Solution on the CHL Cells
10.3969/j.issn.1671-7414.2016.01.032
- VernacularTitle:异种去细胞角膜基质浸提液对 CHL 细胞毒性的实验研究
- Author:
Xianning LIU
;
Yao WANG
;
Xianghua XIAO
;
Shiyin PAN
;
Yong YIN
;
Na AN
;
Xiuping ZHU
- Publication Type:Journal Article
- Keywords:
ostrich acellular corneal stroma;
leaching solution;
cytotoxicity test;
the benchmark non-toxic concentration
- From:
Journal of Modern Laboratory Medicine
2016;(1):112-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To select the largest non-toxic leaching solution concentration through the experimental observation of the cytotoxicity of the ostrich acellular corneal stromal leaching solution to the Chinese hamster lung fibroblasts cells(CHL) for the further chromosome distortion experiment.Methods The leaching solution made from the ostrich acellular corneal stromal material was diluted with concentrate of 1 ∶ 2,1 ∶ 4 and the original concentration were used to culture with the CHL cells,the negative and positive control were also set up at the same time,to evaluate the impact on cell growth after 24 hour by MTT colorimetric method.Results The leaching solution diluted with 1∶4 was non-toxic,and could promote the growth of the cells.Conclusion Combined with the results of classification and cell morphological features,this cytotoxicity test can be used to screen the best benchmark non-toxic concentrations for the chromosome aberration test of the CHL cells.
