Influence of NADH Concetration in the AST Reagent on Assay Performance
10.3969/j.issn.1671-7414.2016.01.035
- VernacularTitle:AST 试剂中 NADH 含量变化对线性范围影响的研究
- Author:
Runqing MU
;
Yinling WANG
;
Qing YAN
- Publication Type:Journal Article
- Keywords:
NADH;
AST;
linear range
- From:
Journal of Modern Laboratory Medicine
2016;(1):121-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To estimate the assay performance of two different kind of reagent with different concentration of NADH.Methods Verificated function index of these two reagents,such as accuracy,sensitiveness,blank space and so on, and determined the molar extinction coefficient of NADH using hexokinase method and calculated the NADH density in two kind of AST reagents.Then estimated the effect of concentation of NADH on the analysis performance.Results NADH concentration was 0.21 mmol/L,and the sample detection limit of the linear was 1 629 U/L.NADH concentration was 0.13 mmol/L,and the sample detection limit of the linear was 1 263 U/L.So the gap between the two reagent’s detection linear was visible.But there were no significant changes in other performance indicators such as detection sensitivity,reagent blank,precision.Conclusion The NADH concentration in the reagent of AST had greatimpact on the detecting linear range, so should pay attention to the potential change of linear range in the daily work.
